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Training workshop on 3D SEM techniques, from sample preparation to image acquisition
November 18, 2024 - 8:00 AM - November 20, 2024 - 5:00 PM
Objectives:
– Acquire the theoretical foundations of sample preparation and 3D SEM imaging.
– Acquire the practical skills and tools needed to prepare biological samples involving high-pressure fixation and rapid cryo-substitution for 3D SEM imaging.
– Attend SEM3D imaging acquisition on a prepared sample, and acquire the skills to align images of a volume after acquisition using FIJI software.
At the end of the course, trainees will be able to :
– define a sample preparation strategy to answer a biological question requiring 3D SEM
– Carry out an A to Z protocol for rapid cryosubstitution (QFS)
– Align a 3D SEM stack