Correlative Microscopy Preserving Native Frozen Sample States
CryoCLEM microscopy combines cryo-electron microscopy (CryoEM) with cryo-light microscopy, enabling the correlation of molecular localization with high-resolution structural details in frozen samples.
This approach preserves the native state of biological samples, providing more accurate insights into their molecular and structural organization.
Applications
CryoCLEM is particularly well-suited for studying biological processes in their native environments. By integrating cryo-light microscopy, researchers can localize specific molecules or regions of interest, which are then further analyzed with cryo-electron microscopy. This seamless workflow ensures precise correlation between molecular localization and ultrastructural details.
Available systems:
At IMM:
The IMM core facility houses state-of-the-art CryoCLEM technology, including all necessary equipment for sample preparation, imaging, and analysis. Additionally, the platform provides:
- Expertise: Guidance from experienced professionals to ensure optimal use of the technology.
- Training: Hands-on sessions to equip users with the skills to conduct CryoCLEM experiments.
- Support: Ongoing assistance to address challenges and refine experimental workflows.
The CryoCLEM platform at PICsL offers a powerful combination of advanced cryo-microscopy technologies and expert support, enabling researchers to explore frozen biological samples with unparalleled precision and accuracy.
Combines cryo-light and cryo-electron microscopy for precise correlations.
Preserves samples' native state for accurate biological insights.
Enables molecular localization with high-resolution structural details.
IMM provides expertise, training, and advanced CryoCLEM technology.
Ideal for studying biological processes in frozen native environments.
