Nanoscale 3D Imaging with SBF-SEM and Micro-CT
Serial Block-Face Scanning Electron Microscopy (SBF-SEM) is a cutting-edge technique designed to produce high-resolution, three-dimensional images of biological and material samples. By integrating automated sectioning with electron microscopy, SBF-SEM enables researchers to explore the ultrastructural details of samples at the nanoscale.
*Visualization and quantification of the amount of contact between mitochondria and plasma membrane (Nicolas BROUILLY and Rémy PUJOL).
Applications
SBF-SEM offers unparalleled capabilities for studying the organization and interaction of complex structures:
- 3D Visualization at High Resolution: Provides nanoscale insights into spatial relationships, essential for fields like neuroscience, materials science, and nanotechnology.
- Efficient Data Collection: Automated sectioning and imaging streamline workflows, making it ideal for large-volume reconstructions.
- Broad Applications: Equally suited for biology and material sciences, it helps unravel intricate structural details across disciplines.
Integration with Micro-CT
Micro-Computed Tomography (micro-CT) is a complementary tool in the SBF-SEM workflow, offering non-destructive imaging for sample preparation. Its key benefits include:
- Precision Targeting: Identifies regions of interest before sectioning, ensuring critical structures are preserved.
- Contextual Analysis: Provides an architectural overview of the sample, later correlated with higher-resolution SBF-SEM images.
- Workflow Optimization: Enhances accuracy and efficiency by guiding the preparation stages and reducing the risk of data loss.
The VolumeEM platform combines SBF-SEM and micro-CT technologies to deliver comprehensive, high-resolution 3D imaging, enabling researchers to unlock new dimensions in their studies.
Correlates micro-CT and SBF-SEM data for comprehensive analysis.
SBF-SEM provides nanoscale 3D imaging of complex structures.
Combines automated sectioning and imaging for efficient data collection.
Micro-CT enables precise targeting during sample preparation.
Ideal for neuroscience, materials science, and nanotechnology applications.
